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This design provides: Enhanced retention of strong basic compounds via cation-exchange mechanism Reverse-phase retention of neutral compounds Additional selectivity for polar molecules, enabling separation of isomers and structurally related compounds Flexible mobile phase compatibility, suitable for LC-MS, preparative chromatography, and low UV detection Typical HPLC Method Parameters Column: Primesep 200, 3.2 150 mm, 5 m, 100 , dual-ended Mobile Phase: MeCN 20% Buffer: Gradient ammonium formate pH 3.0, 2060 mM over 10 min Flow Rate: 0.5 mL/min Detection: LC-MS, ESI, GHK (free peptide, [M+H]+) 341 LOD: 30 ppb *LOD was determined for this specific instrument, method, and analyte

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